ASTM D5712 (Modified Lowry Assay) is a chemical method used to quantify the total extractable protein content of a test item. This method is not specific for latex proteins but will determine the total amount of protein from Hevea brasiliensis and other sources that are present on the test item. NOTE: For this assay, a minimum three items (e.g., gloves) are required for a single test result. ARDL is also accredited for CEN455-3, which is the European Standard equivalent of the ASTM D5712. NOTE: For the CEN procedure, at least 4 gloves are required for testing.